Cloning and Analysis of Partial B Mating Gene Pheromone Receptor in Agrocybe salicacola

机译：Agrocybe Salicacola中部分B交配基因信息素受体的克隆与分析

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The methods of degenerate PCR and DNA walking were used to clone a fragment of 4 274bp nucleotides from A. salicacla strain YAASM0711. With sequence alignment and prediction, one mating gene encoding pheromone receptor was found to be 1194bp nucleotides long and included four introns and five extrons with the lengths of 217bp, 113bp, 67bp, 138bp and 449bp respectively. The spliced open reading frame (ORF) contains 984bp nucleotides encoding 327 amino acid residues, and includes seven transmembrane domains, similar to those from Coprinus cinerea and Laccaria bicolor pheromone receptors. The special primers designed flanking the ORF can separate B1 from B2 mating type strains, indicating that they can be used to distinguish mating types among strains.

机译：退化PCR和DNA步行的方法用于克隆来自A. salicacla菌株Yaasm0711的4274bp核苷酸的片段。随着序列对准和预测，将编码信息素受体的一个配合基因被发现为1194bp核苷酸长，包括四个内含子，其中五个外部，分别为217bp，113bp，67bp，138bp和449bp。拼接开放阅读框（ORF）包含编码327个氨基酸残基的984bp核苷酸，并包括七个跨膜结构域，类似于Coprinus cinerea和曲霉菌双色信息素受体。设计侧翼的特殊引物可以将来自B2配合型菌株的B1分离，表明它们可用于区分菌株之间的配合类型。

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《Congress of the International Society for Mushroom Science》|2012年||共7页
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作者
Weimin Chen; Hongmei Chai; Yongchang Zhao;
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正文语种
中图分类菌类（食用菌）;
关键词
Agrocybe salicacla; Pheromone Receptor; Mating Type;

机译：Agrocybe Salicacla;信息素受体;配合类型;

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专利

1. Molecular cloning and DNA analysis of a gene encoding alpha mating pheromone from the yeast Saccharomyces naganishii [J] . Kodama T, Hisatomi T, Kanemura T, Yeast . 2003,第2期

机译：Naganishii酵母中编码α交配信息素的基因的分子克隆和DNA分析
2. Multiple Genes Encoding Pheromones and a Pheromone Receptor Define the Bβ1 Mating-Type Specificity in Schizophyllum commune [J] . Carlene A. Raper, Charles A. Specht, Lisa J. Vaillancourt, Genetics: A Periodical Record of Investigations Bearing on Heredity and Variation . 1997,第2期

机译：编码信息素和信息素受体的多个基因定义Schizophyllum社中的Bβ1交配型特异性。
3. Roles for Receptors, Pheromones, G Proteins, and Mating Type Genes During Sexual Reproduction in Neurospora crassa [J] . Kim Hyojeong, Wright Sara J., Park Gyungsoon, Genetics: A Periodical Record of Investigations Bearing on Heredity and Variation . 2012,第4期

机译：受体，信息素，G蛋白和交配型基因在有性繁殖中的作用。
4. Cloning and Analysis of Partial B Mating Gene Pheromone Receptor in Agrocybe salicacola [C] . Weimin Chen, Hongmei Chai, Yongchang Zhao Proceedings of the 18th congress of the International Society for Mushroom Science . 2012

机译：沙棘（Agrocybe salicacola）部分B交配基因信息素受体的克隆与分析。
5. Molecular study of diapause via generation of two cDNA libraries and partial cloning of the ecdysone receptor in Sarcophaga crassipalpis [D] . Ball, Elizabeth Nicole 2002

机译：通过产生两个cDNA文库和部分克隆蜕皮虫中蜕皮激素受体的滞育分子研究
6. The mating-type locus B alpha 1 of Schizophyllum commune contains a pheromone receptor gene and putative pheromone genes. [O] . J Wendland, L J Vaillancourt, J Hegner, 1995

机译：Schizophyllum commune的交配型基因座B alpha 1包含信息素受体基因和推定的信息素基因。
7. The mating-type locus B alpha 1 of Schizophyllum commune contains a pheromone receptor gene and putative pheromone genes. [O] . J. Wendland, L. J. Vaillancourt, J. Hegner, 1995

机译：Schizophyllum Commune的交配型基因座Bα1含有能量族酮受体基因和推定的信息素基因。

Cloning and Analysis of Partial B Mating Gene Pheromone Receptor in Agrocybe salicacola

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