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首页> 外文学位 >Chip electrochromatographic systems: Novel vertically aligned carbon nanotube and silica monoliths based separations.
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Chip electrochromatographic systems: Novel vertically aligned carbon nanotube and silica monoliths based separations.

机译：芯片电色谱系统：基于新型垂直排列的碳纳米管和二氧化硅整体的分离。

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Miniaturized chemical analysis systems, also know as 'lab-on-a-chip' devices have been rapidly developing over the last decade. Capillary electrochromatography (CEC), a multidimensional separation technique combining capillary electrophoresis (CE) and liquid chromatography (LC) has been of great interest for chip based applications.;Preliminary work has been undertaken to develop vertically aligned carbon nanotubes and photopolymerizable silica solgel as novel stationary phase materials for 'chip CEC' separations. Patterned growth of CNTs in a specific location of the channel has been carried out using a solid phase Fe- Al catalyst as well as a vapor deposited ferrocene catalyst. Characterization of the CNT "forests" was achieved using optical microscopy, secondary electron microscopy, high resolution tunneling electron microscopy and Raman spectroscopy. Proof-of-concept applications were demonstrated using reversed phase CEC separations as well as solid phase extraction of a glycosylated protein using concanavilin A immobilized onto the CNT bed.;Photopolymerizable silica solgel materials were developed as stationary phase for microfluidic electrochromatographic separations in disposable polydimethylsiloxane (PDMS) chip devices. Effect on morphology and pore size of gels were studied as function of UV and solgel polymerization conditions, porogen, salt additives, geometry and hydrolyzable methoxy-ies. Structural morphologies were studied with Secondary Electron Microscopy (SEM). Pore size and pore volumes were characterized by thermal porometry, nitrogen BET adsorptions and differential scanning calorimetry. Computational fluid dynamics and confocal microscopy tools were employed to study the transport of fluids and model analytes. These investigations were directed towards evolving improved strategies for rinsing of uncrosslinked monomers to form porous monoliths as well as to effect a desired separation under a set of electrochromatograhic conditions. Glycidyloxypropyltrimethoxysilane (GPTMS) mixed with MPTMOS was used as a generic platform to attach affinity ligands such as biotin, boronic acid and cibacron blue to the functionalizable epoxide groups. The binding capacities of target molecules to corresponding affinity ligands were studied by batch binding assays. Preliminary work with corresponding FITC tagged proteins and their interactions with the derivatized and native GPTMOS/MPTMOS under various electrochromatographic conditions have been undertaken.;Research and innovations were also carried out in optical detection systems, hydrophilization of PDMS and induced pressure driven flow systems to support our efforts towards microfluidic chip electrochromatographic systems.
机译：微型化学分析系统，也称为“芯片实验室”设备，在过去十年中发展迅速。毛细管电色谱（CEC）是一种将毛细管电泳（CE）和液相色谱（LC）相结合的多维分离技术，对于基于芯片的应用非常感兴趣;;已经进行了初步工作，以开发垂直排列的碳纳米管和可光聚合的二氧化硅溶胶用于“芯片CEC”分离的固定相材料。已经使用固相Fe-Al催化剂以及气相沉积的二茂铁催化剂进行了在通道的特定位置的CNT的图案化生长。使用光学显微镜，二次电子显微镜，高分辨率隧道电子显微镜和拉曼光谱对CNT“森林”进行了表征。使用反相CEC分离以及使用固定在CNT床上的伴刀豆球蛋白A固相提取糖基化蛋白质，证明了概念验证的应用;开发了可光聚合的二氧化硅溶胶材料作为固定相，用于一次性聚二甲基硅氧烷中的微流体电色谱分离（ PDMS）芯片设备。研究了紫外线和溶胶凝胶聚合条件，成孔剂，盐添加剂，几何形状和可水解甲氧基的作用对凝胶形态和孔径的影响。用二次电子显微镜（SEM）研究了结构形态。孔大小和孔体积通过热孔率法，氮BET吸附和差示扫描量热法进行表征。计算流体动力学和共聚焦显微镜工具用于研究流体的传输和模型分析物。这些研究针对不断发展的改进策略，用于冲洗未交联的单体以形成多孔整料以及在一组电色谱条件下实现所需的分离。缩水甘油基氧丙基三甲氧基硅烷（GPTMS）与MPTMOS混合用作通用平台，以将亲和配体（如生物素，硼酸和西巴龙蓝）连接到可官能化的环氧基上。通过分批结合测定法研究了靶分子与相应亲和配体的结合能力。已进行了相应FITC标记蛋白的初步工作，以及它们在各种电色谱条件下与衍生的GPTMOS /天然GPTMOS / MPTMOS的相互作用的研究;还对光学检测系统，PDMS的亲水化和诱导压力驱动的流动系统进行了研究和创新，以支持我们对微流控芯片电色谱系统的努力。

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