Objectives:To explore and analyze the clinical application of four kinds of test in the detection of gonorrhea neisseria gonorrhoeae.Methods:400 cases of suspected gonorrhea neisseria fungus infection in our hospital from August 2012 to August 2012 were selected and given the following four kinds of detection methods respectively inclu-ding direct smear staining method,cultivating assay method,polymerase reaction method and spot gold immune percola-tion method.The group received cultivating assay method was chosen as control group.The positive detection rate and detection conformity of neisseria gonorrhoeae of the four groups were compared.Results:No significant difference in the positive detection rate was found between the cultivating assay method and spot gold immune percolation method (χ2 =2.89,P >0.05);significant difference in the positive detection rate was found between cultivating assay method and di-rect smear staining method/polymerase reaction method (χ2 =4.35,P <0.05;χ2 =4.57,P <0.05).Cultivating assay method had consistency with polymerase reaction method in the detection of neisseria gonorrhoeae (K =0.890,P <0.05),while cultivating assay method had no consistency with direct smear dyeing method and spot gold immune percola-tion method (P >0.05).Conclusions:At least two detection methods should be adopted in the clinical diagnosis to en-sure the positive detection rate,provides the reasonable basis for the clinical treatment.%目的：探究与分析四种检测法在淋病奈瑟球菌的临床应用。方法：选取我院自2012年8月至2014年8月收治的400例疑似淋病奈瑟菌感染患者作为临床研究对象，分别给予以下四种检测方法包括直接涂片染色法、培养鉴定法、聚合酶连反应法及斑点金免疫渗滤法对标本进行检测，以培养鉴定法作为对照组，对比四组检测方法对淋球菌的阳性检出率以及检测淋球菌的符合度情况。结果：培养鉴定法与斑点金免疫渗滤法阳性检出率相比无显著差异（χ2＝2．89，P ＞0．05）；培养鉴定法分别与直接涂片染色法、聚合酶连反应法阳性检出率相比具有显著差异（χ2＝4．35，P ＜0．05；χ2＝4．57，P ＜0．05）。培养鉴定法与聚合酶连法在检测淋球菌方面存在一致性（K ＝0．890，P ＜0．05），而培养鉴定法与直接图片染色法及斑点金免疫渗滤法均不存在一致性（P ＞0．05）。结论：在临床工作中对微生物进行诊断时建议选择两种以上的检测方法以保证阳性检出率，为临床治疗提供合理依据。
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