通过分析促食欲素A（Orexin A）在牦牛和黄牛消化道不同部位的分布特征及表达差异，探讨Orexin A对牦牛能量代谢的调节作用．运用免疫组织化学SP法检测Orexin A在牦牛和黄牛胃肠道的表达，利用Image‐ProPlus 6.0软件对其表达强度进行半定量分析．结果表明， Orexin A在牦牛和黄牛的皱胃、幽门、贲门和十二指肠均有表达，但表达特征不同；牦牛胃和十二指肠中Orexin A阳性反应产物极显著地低于黄牛（P＜0.01）；牦牛胃肠道中Orexin A阳性反应产物的吸光度分布为：皱胃区＞幽门区＞贲门区＞十二指肠区（P＜0.01）．推测牦牛通过胃肠道Orexin A的低表达，调节中枢及外周组织的激素水平，从而维持机体能量代谢水平，适应青藏高原的特殊环境．%To explore the regulation effect of Orexin A on energy metabolism of yak by analyzing the difference of distribution and expression in gastrointestinal tract between yak and cattle . Immunohistochemistry is used to localize the expression of Orexin A in the gastrointestinal tract of the yak ,Image‐ProPlus 6.0 software is used to semi‐quantitative analyse the intensity of expression of Orexin A . Results show that Orexin A is widely expressed in the abomasum ,pylorus and duodenum cardia in yak and cattle ,which have different expressional characteristics ;The mean absorbance of Orexin A positive reaction product in stomach tissue and duodenum of yak is significantly lower than that of cattle ( P<0.01);By comparing the mean absorbance of Orexin A positive reaction product in the yak , it is found that its expression from strong to weak successively is abomasums area , pyloric region , cardiac region , and then duodenum area (P<0.01) .It indicats that the lower expression of Orexin A could regulate the hormone levels in the central and peripheral tissues to maintain its energy balance to adapt to the extreme environment of the Tibetan plateau .
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