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Natural Red Pigment Production by Monascus Purpureus in Submerged Fermentation Systems Using a Food Industry Waste: Brewer’s Spent Grain

机译:利用食品工业废料在深层发酵系统中生产红曲菌生产天然红色色素:啤酒厂的废谷物

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摘要

This paper studies the production of natural red pigments by Monascus purpureus CMU001 in the submerged fermentation system using a brewery waste hydrolysate, brewer’s spent grain (BSG). The chemical, structural and elemental characterization of the BSG was performed with Van-Soest method, Fourier-transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS), respectively. The lignocellulosic structure of BSG was hydrolyzed with a dilute sulfuric acid solution (2% (w/v)) followed by detoxification with Ca(OH)2. Maximum red pigment production (22.25 UA500) was achieved with the following conditions: 350 rpm shake speed, 50 mL fermentation volume, initial pH of 6.5, inoculation ratio of 2% (v/v), and monosodium glutamate (MSG) as the most effective nitrogen source. Plackett–Burman design was used to assess the significance of the fermentation medium components, and MSG and ZnSO4·7H2O were found to be the significant medium variables. This study is the first study showing the compatibility of BSG hydrolysate to red pigment production by Monascus purpureus in a submerged fermentation system.
机译:本文研究了使用啤酒废渣水解物,啤酒糟(BSG)在深层发酵系统中,红曲霉CMU001生产天然红色颜料的方法。 BSG的化学,结构和元素表征分别通过Van-Soest方法,傅立叶变换红外光谱(FTIR)和X射线光电子能谱(XPS)进行。 BSG的木质纤维素结构用稀硫酸溶液(2%(w / v))水解,然后用Ca(OH)2解毒。在以下条件下实现了最大的红色颜料产量(22.25 UA500):摇动速度350 rpm,发酵体积50 mL,初始pH值为6.5,接种率2%(v / v)和最大的味精(MSG)有效氮源。 Plackett–Burman设计用于评估发酵培养基成分的重要性,发现味精和ZnSO4·7H2O是重要的培养基变量。这项研究是第一个研究表明,BSG水解产物与红曲菌在深层发酵系统中产生的红色素具有相容性。

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