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首页> 外文期刊>Infection and immunity >Interactions of Neisseria gonorrhoeae with human neutrophils: studies with purified PII (Opa) outer membrane proteins and synthetic Opa peptides.
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Interactions of Neisseria gonorrhoeae with human neutrophils: studies with purified PII (Opa) outer membrane proteins and synthetic Opa peptides.

机译：淋病奈瑟氏球菌与人类嗜中性粒细胞的相互作用：用纯化的PII（Opa）外膜蛋白和合成的Opa肽进行研究。

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摘要

We investigated the role of gonococcal outer membrane protein PII (also called Opa protein) in nonopsonic adherence to human neutrophils. Gonococcal outer membranes, purified Opa in detergent (Opa), purified Opa in liposomes (Opa+ lips), and peptides composing the second hypervariable (HV2) region of OpaB (strain FA1090) in liposomes (pepHV2 lips) were tested for their abilities to inhibit subsequent gonococcal adherence to human neutrophils. Outer membranes from gonococci possessing adherent Opa, liposomes containing adherent Opa, purified adherent Opa, and two of three liposome preparations (pepHV2 lips) containing peptides from the HV2 region of an adherent Opa inhibited subsequent adherence to neutrophils of homologous Opa+ gonococci. On the other hand, outer membranes from Opa- gonococci, outer membranes containing a nonadherent Opa (OpaA from strain FA1090), purified OpaA, and OpaA lips had little or no inhibitory effect. Outer membranes containing adherent Opas, purified adherent Opas, and liposomes containing such Opas all bound to neutrophils, whereas preparations containing OpaA or no Opa protein did not. The results indicate that (i) Opa proteins can bind to neutrophils in a partially purified or purified form and (ii) the HV2 region of Opa appears to at least partially mediate Opa's biological role.
机译：我们调查了淋球菌外膜蛋白PII（也称为Opa蛋白）在非调理性人类嗜中性粒细胞粘附中的作用。测试了淋球菌外膜，去污剂（Opa）中纯化的Opa，脂质体（Opa +嘴唇）中纯化的Opa以及脂质体（pepHV2嘴唇）中组成OpaB第二高变区（HV1090）的肽（FA1090菌株）的肽。随后淋球菌对人中性粒细胞的依从性。淋球菌的外膜具有粘附的Opa，含有粘附的Opa的脂质体，纯化的粘附的Opa以及含有来自粘附的Opa HV2区的肽的三种脂质体制剂（pepHV2唇）中的两个抑制了随后对同源Opa +淋球菌的中性粒细胞的粘附。另一方面，来自Opagonococci的外膜，包含非粘附性Opa（来自FA1090菌株的OpaA），纯化的OpaA和OpaA嘴唇的外膜几乎没有抑制作用。含有粘附性Opas的外膜，纯化的粘附性Opas和含有此类Opas的脂质体均与中性粒细胞结合，而含有OpaA或不含Opa蛋白的制剂则没有。结果表明：（i）Opa蛋白可以部分纯化或纯化的形式与嗜中性白细胞结合，（ii）Opa的HV2区似乎至少部分介导了Opa的生物学作用。

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