The challenge of enumerating Listeria monocytogenes in food

Anais Auvolat; Nathalie Gnanou Besse

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The challenge of enumerating Listeria monocytogenes in food

机译：列举食品中单核细胞增生李斯特菌的挑战

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Listeria monocytogenes is recognised as a serious foodborne pathogen in humans. However, food products are usually contaminated at low levels (i.e. ＜100 CFU/g) and there is still no adequate enumeration method for testing food. Much research has been carried out to improve Listeria enumeration methods, leading to several proposed alternative methods such as the most probable number technique, molecular-based methods and bacterial cell concentration techniques. Here, we catalogue the current knowledge concerning L. monocytogenes enumeration, with a particular focus on the problem of enumerating low level contamination.

机译：单核细胞增生性李斯特菌被认为是人类的严重食源性病原体。然而，食品通常被低水平污染（即<100 CFU / g），并且仍然没有足够的枚举方法来测试食品。为了改进李斯特菌计数方法，已经进行了许多研究，从而提出了几种提议的替代方法，例如最可能的数技术，基于分子的方法和细菌细胞浓缩技术。在这里，我们将有关单核细胞增生李斯特氏菌计数的当前知识编目，特别着重于枚举低水平污染的问题。

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来源
《Food microbiology》 |2016年第febaptab期|135-149|共15页
作者
Anais Auvolat; Nathalie Gnanou Besse;
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作者单位

French Agency for Food, Environmental and Occupational Health & Safety (ANSES), Laboratory for Food Safety, ANSES, Laboratoire de Securite des Aliments, 14 Rue Pierre et Marie Curie, 94701 Maisons Alfort, France;

French Agency for Food, Environmental and Occupational Health & Safety (ANSES), Laboratory for Food Safety, ANSES, Laboratoire de Securite des Aliments, 14 Rue Pierre et Marie Curie, 94701 Maisons Alfort, France;

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收录信息美国《科学引文索引》(SCI);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类
关键词
Listeria monocytogenes; Microbiological methods; Enumeration; MPN; Quantitative PCR; Filtration; Digital PCR;

机译：李斯特菌;微生物学方法;列举;MPN;定量PCR;过滤;数字PCR;

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专利

1. Prevalence, enumeration, and pheno- and genotypic characteristics of Listeria monocytogenes isolated from raw foods in South China [J] . Moutong Chen, Qingping Wu, Jumei Zhang, Frontiers in Microbiology . 2015,第6期

机译：从华南地区生食中分离出的单核细胞增生李斯特菌的流行，计数以及表型和基因型特征
2. Detection and enumeration of viable Listeria monocytogenes cells from ready-to-eat and processed vegetable foods by culture and DVC-FISH. [J] . Moreno Y, Sanchez-Contreras J, Montes R. M, Food Control . 2012,第2期

机译：通过培养和DVC-FISH检测和计数即食和加工蔬菜食品中活的单核细胞增生李斯特菌细胞。
3. Validation of NMKL method No. 136 Listeria monocytogenes, detection and enumeration in foods and feed. [J] . Loncarevic S., Okland M., Sehic E., International Journal of Food Microbiology . 2008,第2期

机译：验证NMKL第136号单核细胞增生李斯特氏菌方法，食品和饲料中的检测和计数。
4. Detection of Listeria monocytogenes in foods by SYBR Green I melting curve [C] . HOU Hong-mana, CUI Yu-na, ZHANG Gong-liang International Conference on Chemical Engineering and Advanced Materials . 2013

机译：Sybr Green I熔化曲线检测食品中李斯特里亚单核细胞增生
5. Role of lactic acid bacteria as a bio-sanitizer to prevent attachment of Listeria monocytogenes on deli slicer contact surfaces, and the influence of Listeria innocua on the attachment of L. monocytogenes on selected materials located in the food-processing environment. [D] . Ndahetuye, Jean Baptiste. 2012

机译：乳酸菌作为生物消毒剂的作用，可防止单核细胞增生李斯特菌附着在熟食切片机的接触表面上，以及无毒李斯特菌对单核细胞增生李斯特氏菌附着在食品加工环境中所选材料上的影响。
6. Prevalence, enumeration, and pheno- and genotypic characteristics of Listeria monocytogenes isolated from raw foods in South China [O] . Moutong Chen, Qingping Wu, Jumei Zhang, 2020

机译：从华南地区生食中分离出单核细胞增生李斯特菌的流行，计数以及表型和基因型特征
7. Prevalence, enumeration and pheno- and genotypic characteristics of Listeria monocytogenes isolated from raw foods in South China [O] . Moutong eChen, Qingping eWu, Jumei eZhang, 2015

机译：华南地区生食单核细胞增生李斯特菌的流行，计数及表型和基因型特征

The challenge of enumerating Listeria monocytogenes in food

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