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Development of an alternative culture medium for the selective enumeration of Lactobacillus casei in fermented milk

机译：开发用于选择性计数发酵乳中干酪乳杆菌的替代培养基

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Monitoring the populations of probiotic strains of the species Lactobacillus casei in food is required by food industries in order to assure that a minimum concentration of these organisms will be ingested by consumers. In this context, Petrifilm™ AC plates can be used along with selective culture media to allow the enumeration of specific groups of lactic acid bacteria. The present study aimed to assess chemical substances as selective agents for Lb. casei in order to propose a selective culture medium to be used with Petrifilm™ AC plates as an alternative protocol for the enumeration of probiotic strains of this species in fermented milk. Twenty-six probiotic and starter cultures (including six strains of Lb. casei) were plated on de Man Rogosa and Sharpe (MRS) agar with distinct concentrations of nalidixic acid, bile, lithium chloride, metronidazole, sodium propionate, and vancomycin. Vancomycin at 10 mg/L demonstrated selective activity for Lb. casei. In addition, 2,3,5-triphenyltetrazolium chlorine was identified as a compound that did not inhibit Lb. casei, and Petrifilm™ AC plates used with MRS and vancomycin at 10 mg/L (MRS-V) demonstrated more colonies of this organism when incubated under anaerobic conditions than aerobic conditions. Acidophilus milk and yoghurt were prepared, added to Lb. casei strains, and stored at 4℃. Lb. casei populations were monitored using MRS-V and MRTLV by conventional plating and associated with Petrifilm™ AC plates. All correlation indices between counts obtained by conventional plating and Petrifilm™ AC were significant (p ＜ 0.05), but the best performance was observed for growth on MRS-V. The obtained data indicate the efficiency of using MRS-V associated with Petrifilm™ AC plates for the enumeration of Lb. casei strains in fermented milk. However, the selective potential of this culture medium must be evaluated considering the specific strains of Lb. casei and the starter cultures inoculated in the fermented milk that requires monitoring.
机译：食品工业需要监测食品中干酪乳杆菌种的益生菌菌株的种群，以确保消费者摄入这些生物的最低浓度。在这种情况下，可以将Petrifilm™AC板与选择性培养基一起使用，以列举出特定种类的乳酸菌。本研究旨在评估化学物质作为Lb的选择剂。 casei为了提出一种选择性培养基与Petrifilm™AC板一起使用，作为在发酵乳中枚举该物种的益生菌菌株的替代方案。将26种益生菌和发酵剂培养物（包括6株干酪乳杆菌）接种在含有不同浓度的萘啶酸，胆汁，氯化锂，甲硝唑，丙酸钠和万古霉素的de Man Rogosa和Sharpe（MRS）琼脂上。 10 mg / L的万古霉素显示出对Lb的选择性活性。凯西。另外，鉴定出2,3,5-三苯基四唑氯为不抑制Lb的化合物。 Casei和10 mg / L（MRS-V）的MRS和万古霉素一起使用的Petrifilm™AC平板在无氧条件下培养的菌落要比有氧条件下的菌落多。制备了嗜酸性牛奶和酸奶，添加到Lb中。干酪菌株，并在4℃下保存。磅。使用MRS-V和MRTLV通过常规平板并与Petrifilm™AC平板相关联来监测酪蛋白种群。通过常规电镀和Petrifilm™AC获得的计数之间的所有相关指数均很显着（p ＜0.05），但在MRS-V上观察到了最佳生长性能。获得的数据表明使用与Petrifilm™AC板关联的MRS-V进行Lb枚举的效率。发酵乳中的干酪素菌株。但是，必须考虑Lb的特定菌株来评估这种培养基的选择性潜力。干酪乳和发酵乳中接种的起子培养物需要监测。

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