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首页> 外文期刊>Food research international >Isolation, molecular and phenotypic characterization of Cronobacter spp. in ready-to-eat salads and foods from Japanese cuisine commercialized in Brazil
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Isolation, molecular and phenotypic characterization of Cronobacter spp. in ready-to-eat salads and foods from Japanese cuisine commercialized in Brazil

机译：克氏杆菌属的分离，分子和表型表征。即食沙拉和在巴西商业化的日本料理中的食物

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The aim of this study was to detect Cronobacter from 30 samples of ready-to-eat (RTE) salads and 30 foods from Japanese cuisine as commercially available in Brazil. The detection of Cronobacter was as according to the ISO standard 22964:2017. The isolates were phenotypically characterized by Vitek 2.0 and the antibiotic susceptibility profile was determined using the standardized agar disc diffusion method. Molecular characterization was accomplished by real-time PCR targeting dnaG gene, multiplex-PCR targeting cgcA gene, and fusA allele sequencing. Twenty-seven samples (45.0%) contained Cronobacter, 14 (23.3%) samples of foods from Japanese cuisine and 13 (21.7%) samples of RTE salads. Twenty-nine unique Cronobacter isolates were selected from the 27 positive samples and were identified as C. sakazakii (n = 18), C. maloncuicus (n = 8), and C. dublinensis (n = 3). A high genetic diversity was observed, with 29 Cronobacter strains being assigned to 11 different fusA alleles, a ratio of 2.6 strains by fusA allele was found. The cgcA multiplex-PCR failed to identify many of the Cronobacter isolates at the species level. Four (13.8%) Cronobacter isolates were resistant to one or more antibiotics tested (n = 12). The presence of Cronobacosr in RTE foods could be a potential threat to human health and highlights the need for high levels of hygiene, particularly when preparing food for elderly, immunosuppressed persons or adults with prior underlying pathology. Epidemiological surveillance agencies should be aware of the risk that these RTE foods may represent, for these groups.
机译：这项研究的目的是从30份即食（RTE）色拉样品和30种在日本可商购的日本料理食品中检测Cronobacter。克罗诺杆菌的检测符合ISO标准22964：2017。通过Vitek 2.0在表型上对分离物进行了表征，并使用标准化的琼脂圆盘扩散法确定了抗生素敏感性。通过靶向dnaG基因的实时PCR，靶向cgcA基因的多重PCR和fusA等位基因测序来完成分子表征。二十七份样品（45.0％）包含克罗诺杆菌，14种（23.3％）日本料理食品样品和13种（21.7％）RTE色拉样品。从27个阳性样品中选择了29个独特的克罗诺杆菌分离株，并将其鉴定为阪崎假丝酵母（n = 18），丙酸假丝酵母（n = 8）和都柏林假丝酵母（n = 3）。观察到高遗传多样性，将29个克氏杆菌菌株分配给11个不同的fusA等位基因，发现2.6菌株的fusA等位基因比例。 cgcA多重PCR未能在物种水平上鉴定出许多克隆杆菌。四种（13.8％）克罗诺杆菌分离株对一种或多种测试的抗生素具有抗性（n = 12）。 RTE食品中存在Cronobacosr可能对人类健康构成潜在威胁，并突出显示了对高水平卫生的需求，特别是在为具有潜在基础病状的老年人，免疫抑制患者或成人制备食品时。流行病学监测机构应意识到这些RTE食品可能对这些人群造成的风险。

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