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首页> 外文期刊>Pigment cell research >Photobleaching of retinal pigment epithelium melanosomes reduces their ability to inhibit iron-induced peroxidation of lipids
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Photobleaching of retinal pigment epithelium melanosomes reduces their ability to inhibit iron-induced peroxidation of lipids

机译：视网膜色素上皮黑素体的光漂白降低了它们抑制铁引起的脂质过氧化的能力

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摘要

Melanin in the human retinal pigment epithelium (RPE) is believed to play an important photoprotective role. However, unlike in skin, melanosomes in the RPE are rather long-lived organelles, which increases their risk of modifications resulting from significant fluxes of light and high oxygen tension. In this work, we subjected purified bovine RPE melanosomes to prolonged aerobic exposure with intense visible and near ultraviolet radiation and studied the effects of irradiation on the melanosome's capacity to inhibit peroxidation of lipids induced by iron/ascorbate. We found that control, untreated melanosomes show a concentration-dependent inhibition of the accumulation of lipid hydroperoxides and the accompanying consumption of oxygen, but photolysed melanosomes lose their antioxidant efficiency and even became prooxidant. The prooxidant action of partially photobleached melanosomes was observed for pigment granules with a melanin content reduced by about 50% compared with untreated melanosomes, as determined by electron spin resonance spectroscopy. We have previously shown that a similar loss in the content of the RPE melanin occurs during human lifetime, which may suggest that the normal antioxidant properties of human RPE melanin become compromised with aging.
机译：人们认为人视网膜色素上皮（RPE）中的黑色素起着重要的光保护作用。但是，与皮肤不同，RPE中的黑素体是相当长寿的细胞器，这会增加它们因大量光通量和高氧张力而被修饰的风险。在这项工作中，我们对纯化的牛RPE黑素体进行了长时间的有氧暴露，并接受了强烈的可见光和近紫外线辐射，并研究了辐射对黑素体抑制铁/抗坏血酸诱导的脂质过氧化能力的影响。我们发现未经处理的对照黑素体对脂质氢过氧化物的积累和氧气的消耗表现出浓度依赖性的抑制作用，但是光解黑素体失去了其抗氧化效率，甚至变成了抗氧化剂。通过电子自旋共振光谱法测定，与未处理的黑体相比，对于黑色素含量减少约50％的颜料颗粒，观察到部分光漂白的黑体的促氧化作用。先前我们已经表明，在人类一生中RPE黑色素含量发生了类似的损失，这可能表明人类RPE黑色素的正常抗氧化特性会随着衰老而受到损害。

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