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首页> 外文期刊>Journal of genetics >Genomewide analysis of intronic microRNAs in rice and Arabidopsis
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Genomewide analysis of intronic microRNAs in rice and Arabidopsis

机译：水稻和拟南芥中内含子microRNA的全基因组分析

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摘要

MicroRNAs (miRNAs) are potent regulators of gene transcription and posttranscriptional processes. The majority of miRNAs are localized within intronic regions of protein-coding genes (host genes) and have diverse functions in regulating important cellular processes in animals. To date, few plant intronic miRNAs have been studied functionally. Here we report a comprehensive computational analysis to characterize intronic miRNAs in rice and Arabidopsis. RT-PCR analysis confirmed that the identified intronic miRNAs were derived from the real introns of host genes. Interestingly, 13 intronic miRNAs in rice and two in Arabidopsis were located within seven clusters, of which four polycistronic clusters contain miRNAs derived from different families, suggesting that these clustered intronic miRNAs might be involved in extremely complex regulation in rice. Length analysis of miRNA-carrying introns, promoter prediction and qRT-PCR analysis results indicated that intronic miRNAs are coexpressed with their host genes. Expression pattern analysis demonstrated that host genes had a very broad expression spectrum in different stages of development, suggesting the intronic miRNAs might play an important role in plant development. This comparative genomics analysis of intronic miRNAs in rice and Arabidopsis provides new insight into the functions and regulatory mechanisms of intronic miRNAs in monocots and dicots.
机译：MicroRNA（miRNA）是基因转录和转录后过程的有效调节剂。大多数miRNA位于蛋白质编码基因（宿主基因）的内含子区域，在调节动物重要细胞过程中具有多种功能。迄今为止，几乎没有功能性研究植物内含子miRNA。在这里，我们报告了全面的计算分析，以鉴定水稻和拟南芥中的内含子miRNA。 RT-PCR分析证实，鉴定出的内含子miRNA来源于宿主基因的真正内含子。有趣的是，水稻中的13个内含子miRNA和拟南芥中的2个位于七个簇中，其中四个多顺反子簇包含来自不同家族的miRNA，这表明这些簇状的内含子miRNA可能参与了水稻极其复杂的调控。携带miRNA的内含子的长度分析，启动子预测和qRT-PCR分析结果表明，内含子miRNA与它们的宿主基因共表达。表达模式分析表明，宿主基因在发育的不同阶段具有非常广泛的表达谱，表明内含子miRNA可能在植物发育中发挥重要作用。水稻和拟南芥中内含子miRNA的这种比较基因组学分析为单子叶植物和双子叶植物中内含子miRNA的功能和调控机制提供了新见解。

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