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首页> 外文期刊>Acta biomaterialia >Preparation of stem cell aggregates with gelatin microspheres to enhance biological functions.
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Preparation of stem cell aggregates with gelatin microspheres to enhance biological functions.

机译：用明胶微球制备干细胞聚集体，以增强生物学功能。

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The objective of this study is to improve the viability and osteogenic differentiation of cultured rat bone marrow-derived mesenchymal stem cells (MSC) by the use of gelatin hydrogel microspheres. Gelatin was dehydrothermally crosslinked at 140 degrees C for 48 h in a water in oil emulsion state. When cultured with the gelatin hydrogel microspheres in round, U-bottomed wells of 96-well plates coated with poly(vinyl alcohol) MSC formed aggregates homogeneously incorporating the microspheres. The viability of the cell aggregates was significantly higher compared with that of aggregates formed without microspheres. MSC proliferation in the aggregates depended on the number and diameter of the incorporated microspheres. Higher MSC proliferation was observed for aggregates incorporating a greater number of larger gelatin microspheres. When evaluated as a measure of aerobic glycolysis the ratio of l-lactic acid production/glucose consumption in MSC was significantly lower for MSC cultured with gelatin microspheres than those without microspheres. MSC production of alkaline phosphatase (ALP) and sulfated glycosaminaglycan (sGAG) was examined to evaluate their potential osteogenic and chondrogenic differentiation. The amount of ALP produced was significantly higher for MSC aggregates cultured with gelatin microspheres than that of MSC cultured without microspheres. On the other hand, the amount of sGAG produced was significantly lower for MSC aggregates containing microspheres. It is concluded that the incorporation of gelatin hydrogel microspheres prevents the aggregated MSC suffering from a lack of oxygen, resulting in enhanced MSC aggregation and cell proliferation and osteogenic differentiation.
机译：这项研究的目的是通过使用明胶水凝胶微球改善培养的大鼠骨髓间充质干细胞（MSC）的活力和成骨分化。将明胶在油乳液状态下的水中于140摄氏度下进行脱氢热交联48小时。当与明胶水凝胶微球一起培养时，涂有聚乙烯醇MSC的96孔板的U形底孔形成均匀聚集了微球的聚集体。与没有微球形成的聚集体相比，细胞聚集体的活力明显更高。聚集体中的MSC增殖取决于所掺入的微球的数量和直径。对于包含大量较大的明胶微球的聚集体，观察到较高的MSC增殖。当评估为有氧糖酵解的量度时，用明胶微球培养的MSC的MSC中l-乳酸产生/葡萄糖消耗的比率显着低于无微球培养的MSC。检查了碱性磷酸酶（ALP）和硫酸化糖胺聚糖（sGAG）的MSC产生，以评估其潜在的成骨和软骨形成分化。用明胶微球培养的MSC聚集体产生的ALP量明显高于不使用微球培养的MSC。另一方面，对于含有微球的MSC聚集体，产生的sGAG量明显较低。结论是，掺入明胶水凝胶微球可防止聚集的MSC缺氧，从而增强MSC的聚集，细胞增殖和成骨分化。

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