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首页> 外文期刊>International Journal of Food Microbiology >Efficacy of chlorine, acidic electrolyzed water and aqueous chlorine dioxide solutions to decontaminate Escherichia coli O157:H7 from lettuce leaves.
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Efficacy of chlorine, acidic electrolyzed water and aqueous chlorine dioxide solutions to decontaminate Escherichia coli O157:H7 from lettuce leaves.

机译：氯，酸性电解水和二氧化氯水溶液对生菜叶片中大肠杆菌O157：H7的去污效果。

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This study compared the efficacy of chlorine (20-200 ppm), acidic electrolyzed water (50 ppm chlorine, pH 2.6), acidified sodium chlorite (20-200 ppm chlorite ion concentration, SanovaReg.), and aqueous chlorine dioxide (20-200 ppm chlorite ion concentration, TriNovaReg.) washes in reducing populations of Escherichia coli O157:H7 on artificially inoculated lettuce. Fresh-cut leaves of Romaine or Iceberg lettuce were inoculated by immersion in water containing E. coli O157:H7 (8 log CFU/ml) for 5 min and dried in a salad spinner. Leaves (25 g) were then washed for 2 min, immediately or following 24 h of storage at 4 degrees C. The washing treatments containing chlorite ion concentrations of 100 and 200 ppm were the most effective against E. coli O157:H7 populations on Iceberg lettuce, with log reductions as high as 1.25 log CFU/g and 1.05 log CFU/g for TriNovaReg. and SanovaReg. wash treatments, respectively. All other wash treatments resulted in population reductions of less than 1 log CFU/g. Chlorine (200 ppm), TriNovaReg., SanovaReg., and acidic electrolyzed water were all equally effective against E. coli O157:H7 on Romaine, with log reductions of approx. equal to 1 log CFU/g. The 20 ppm chlorine wash was as effective as the deionized water wash in reducing populations of E. coli O157:H7 on Romaine and Iceberg lettuce. Scanning electron microscopy indicated that E. coli O157:H7 that was incorporated into biofilms or located in damage lettuce tissue remained on the lettuce leaf, while individual cells on undamaged leaf surfaces were more likely to be washed away
机译：这项研究比较了氯（20-200 ppm），酸性电解水（50 ppm氯，pH 2.6），酸化亚氯酸钠（20-200 ppm亚氯酸盐离子浓度，SanovaReg。）和二氧化氯水溶液（20-200）的功效。 ppm的亚氯酸根离子浓度（TriNovaReg。）会在人工接种的生菜上减少大肠杆菌O157：H7的数量。将长叶莴苣或卷心莴苣的鲜切叶接种到含大肠杆菌O157：H7（8 log CFU / ml）的水中浸泡5分钟，然后在沙拉旋转器中干燥。然后将叶子（25 g）洗净2分钟，即刻或在4摄氏度下存放24小时后洗净。含有亚氯酸根离子浓度分别为100和200 ppm的洗净处理对冰山上的O157：H7大肠杆菌群最有效生菜，TriNovaReg的对数减少量高达1.25 log CFU / g和1.05 log CFU / g。和SanovaReg。分别洗涤。所有其他洗涤处理导致种群减少量小于1 log CFU / g。氯（200 ppm），TriNovaReg。，SanovaReg。和酸性电解水对长叶莴苣中的大肠杆菌O157：H7均具有同等效力，对数减少约0.12。等于1 log CFU / g。在减少长叶莴苣和卷心莴苣上的大肠杆菌O157：H7种群时，用20 ppm氯洗涤与去离子水洗涤一样有效。扫描电子显微镜表明，掺入生物膜或位于受损莴苣组织中的大肠杆菌O157：H7保留在莴苣叶片上，而未受损叶片表面上的单个细胞更可能被洗掉

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