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遗传监测

遗传监测的相关文献在1990年到2018年内共计71篇,主要集中在动物学、基础医学、遗传学 等领域,其中期刊论文65篇、会议论文5篇、专利文献269669篇;相关期刊30种,包括四川动物、遗传、中国实验动物学报等; 相关会议5种,包括2011年第9届中国北方实验动物科技年会、2010广州-东莞首届国际小型猪学术论坛、第九届中国实验动物科学年会等;遗传监测的相关文献由218位作者贡献,包括李瑞生、陈振文、王纯耀等。

遗传监测—发文量

期刊论文>

论文:65 占比:0.02%

会议论文>

论文:5 占比:0.00%

专利文献>

论文:269669 占比:99.97%

总计:269739篇

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遗传监测

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  • 李瑞生
  • 陈振文
  • 王纯耀
  • 杨卫红
  • 宋国英
  • 谢建云
  • 刘双环
  • 战大伟
  • 王承利
  • 魏泓
  • 期刊论文
  • 会议论文
  • 专利文献

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    • 韩琳; 谢建云; 杨斐; 胡樱; 田立立; 鲍世民
    • 摘要: Objective To analyze the single nucleotide polymorphism (SNP) genotyping of inbred mice from Shanghai.Methods 44 SNP loci on 21 chromosomes of 10 inbred mice strains from 2 laboratory animal companies of Shanghai were detected by the PCR-LDR genotyping panels,and the other 2 inbred mice strains were selected as the double blind samples for verification.And discrepancy locus were verified by sequencing.Results The SNP genotyping results of all the samples from the same inbred mice strains were homomorphism,but 6 inbred mice strains from different populations (BALB/c,FVB,DBA/2 and C3H/He) were polymorphism with 7 and 2 discrepancy locus respectively.The sequencing results to verify the discrepancy locus were the same as the SNP genotyping results.Conclusions The SNP genotyping was very stable and consistent in inbred mice strains from the same population in Shanghai,and the SNP genotyping of the same inbred mice strains from different populations was polymorphism.%目的 比较上海地区常用近交系小鼠单核苷酸多态性(SNP)位点分型情况.方法 采用多重PCR-LDR检测技术,选取上海地区2家单位的10个近交系小鼠,对21条染色体上的44个SNP位点进行分型检测,同时,随机选取上海地区2个近交系品系作为双盲样本,对分型方案进行验证,差异位点利用测序法进行验证.结果 同一来源的10个近交系小鼠,同品系间SNP分型结果完全一致;不同种群来源6个品系SNP分型结果提示,BALB/c,FVB,DBA/2和C3H/He在44个位点上分型结果均相同,CBA和C57BL/6分别在7个和2个位点存在差异;差异位点经测序验证结果与SNP分型方案一致.结论 上海地区常用近交系小鼠中,同一来源的品系中SNP遗传质量具有良好稳定性和一致性,不同种群来源的相同品系间SNP分型存在差异.
    • 李迎; 杜小燕; 陈振文; 马兰芝; 尚世臣; 尚玉璞; 赵权; 李桂军; 崔晓霞; 王冬平
    • 摘要: 目的 建立近交系长爪沙鼠生化标记遗传检测方法,开展对长爪沙鼠脑缺血模型CMU/1和CMU/2近交系的纯合度进行分析,为判定其遗传状况提供依据.方法 采用醋酸纤维板电泳法,选26个生化标记进行近交系长爪沙鼠各组织器官样品电泳,参考前期方法优化最佳电泳条件,并对样品处理和染色的方法进行一定的改良后;针对F21~23代77只CMU/1和44只CMU/2近交系动物26个生化标记的纯合度进行检测.结果 在2个近交系长爪沙鼠共121只动物中均能成功检测到26个生化标记.其中有24个位点在CMU/1和CMU/2品系内和品系间均显示单态性,但是Es-3和Es-4位点在2个品系间有差异.结论 建立了长爪沙鼠近交系生化标记遗传检测方法;确定近交系CMU/1和CMU/2动物26个生化标记,其纯合度达到100%,说明2个近交系品系符合近交系动物的标准(GB14923-2010).%Objective To establish genetic monitoring method by biochemical markers for inbred gerbils and apply it in analyzing the homogenous of the ischemia-prone inbred gerbil lines CMU/1 and CMU/2.Methods Twenty-six biochemical marker loci were selected to perform cellulose acetate fiber electrophoresis for several kinds of gerbil tissues by optimum electrophoresis conditions referred to previous report and optimized the sample treatment and staining method.Then these methods were used in detecting homogeneous of 2 inbred lines CMU/1 (77 gerbils) and CMU/2 (44 gerbils) genetic quality involved generation F21-F23.Results All of 26 biochemical marker loci could be detected successfully in both inbred gerbil of 121 gerbils.Thereinto,24 loci exhibited monomorphism within and between CMU/1 and CMU/2.However,the loci Es-3 and Es-4 showed polymorphism between two strains.Conclusion The biochemical marker method for genetic monitoring of inbred gerbil has been successfully established.The 26 biochemical marker loci for inbred gerbil strain CMU/1 and CMU/2 has been confirmed which homogeneous reached to 100%.These data indicated that two inbred strains match the standard of inbred laboratory animals (GB14923-2010).
    • 房晓欢; 尹彦开; 刘洋洋; 贾青; 尹雪姣; 赵思思; 李赛
    • 摘要: A method was created to reduce the sampling number in order to make the preservation monitoring simpler and accurate for the Shenxian pigs' population.A total of 179 Shenxian pigs as a population was sampled in a certain gradient,each gradient randomly selected 10 times.The genetic diversity indexes of population and sampling groups were calculated using microsatellite primers marker technique.The average and standard deviation of the genetic diversity indexs of 10 groups were compared with that of the selected population,and the minimum sampling size with less changes in genetic diversity was determined as the appropriate sampling scale.The results showed that:For the group with sampling size of 30,the average allele number was 4.6609,the average effective allele number was 3.2325,the average observed heterozygosity was 0.1569,the average expected heterozygosity was 0.6284,the average PIC was 0.5794.The results indicated that the main indexes of genetic diversity in group with sampling size of 30 were similar to those in the whole population,so the sampling size of 30 pigs could be used to represent and instead of large population of Shenxian pigs.%为使深县猪保种效果监测更为简化又不失准确性,特建立一种方法以缩减深县猪采样的数量.选取179头深县猪作为一个群体,按照一定的数量梯度抽样,每个梯度随机抽取10次,采用微卫星引物标记的技术计算群体与抽样群的遗传多样性指标.综合10组的遗传多样性评价指标的平均值和标准差与选取的群体对应数值进行比较分析,以遗传多样性变化最小的抽样规模确定为适宜采样规模.结果显示,抽样规模为30头的深县猪遗传多样性主要数据为:平均等位基因数4.6609,平均有效等位基因数3.2325,平均期望杂合度0.6284,平均观察杂合度0.1569,平均PIC 0.5794,与整体数据相比,变化较小,具有代表性,可以代替大群测定.
    • 王龙霞; 赵先哲; 乔伟伟
    • 摘要: 自发性模型动物是研究糖尿病、高血压和肥胖等疾病的有力工具,但是这些特殊的动物模型容易受环境及饮食的影响而产生变异.本文通过简要的诠释代谢组学这一新型研究手段,介绍这种方法在发掘特殊模型动物生物标志的应用,引出其用于监测特殊模型动物遗传质量的可行性和开发专门适用于这些特殊模型动物的饲料的必要性和前景,为以后的研究提供参考.
    • 魏杰; 王洪; 李芳芳; 岳秉飞
    • 摘要: Objective To analyse and monitor the genetic quality of closed colony NIH mice in Beijing district for the last 3 years.Methods We use biochemical genetic markers( including alkaline phosphatase -1 and the like 14 biochemical markers), selecting A and B colonies from different facilities for genetic monitoring in 2011 to study the polymorphism.And in 2014, 30 NIH mice just from B colony were monitored using the same testing and sampling methods.Results In 2011,NIH mice form both A and B facilities existed 6 polymorphic biochemical markers(Ce2,Car2, Gpi1,Es10,Gpd1,Pgm1); and NIH mice of B company also existed polymorphism in Es3 loucs.Between the 2 NIH mice colonies, there were significant difference in Es3、Gpd1、Pgm1 loci (P <0.01), and difference in Car2 locus(P <0.05).FST of the 2 colonies was 0.0406, the genetic identity was 0.9619, and the genetic distance was 0.0388.In B company, NIH mice of 2014 appeared 2 homozygous loci(Ce2 and Gpd1) when compared with NIH mice of 2011.Between the 2 NIH mice colonies, there were significant difference in Es10 and Gpd1 loci (P <0.01), and difference in Pgm1 locus(P <0.05).Fst of the 2 colonies was 0.1103, the genetic identity was 0.8847, and the Genetic distance was 0.1266.Conclusions Population isolation, breeding and selection, populationpopulation quantityquantity and generation significantly affected the genetic architecture of NIH mice.So when breeding and reserving seeds, we should strengthen the genetic monitoring of outbred NIH mice, in order to offer reliable genetic quality protection.%目的:对近3年北京地区的两个 NIH 封闭群小鼠群体的遗传质量进行监测分析。方法利用生化标记基因检测法,2011年测定 A、B 两单位 NIH 小鼠在碱性磷酸酶-1等14个遗传生化标记位点上的多态性。2014年利用相同方法原则对 B 单位的 NIH 小鼠群体进行抽样检测,比较了该小鼠群体3年来的遗传构成变化。结果2011年,A、B 两单位 NIH 小鼠群体均呈多态性的生化标记位点有6个(Ce2、Car2、Gpi1、Es10、Gpd1、Pgm1),且 B 单位在 Es3位点也呈多态性;两群 NIH 小鼠在 Car2位点有差异(P <0.05),在 Es3、Gpd1、Pgm1三个位点有显著差异(P <0.01);两群体的群间分化系数为0.0406,遗传一致性指数为0.9619,遗传距离为0.0388。与2011年相比,B 单位封闭群 NIH 小鼠在2014年出现了2个纯合位点(Ce2和 Gpd1),同时 Es10和 Gpd1两位点差异极显著(P <0.01),Pgm1位点差异显著(P <0.05);不同代次 NIH 小鼠群间分化系数为0.1103,遗传一致性指数为0.8847,遗传距离为0.1266。结论群体隔离、选种育种、种群数量和繁育代次等对 NIH 小鼠遗传构成差异影响显著。留种和繁育生产时应加强封闭群 NIH 小鼠的遗传监测,为其遗传质量的稳定性提供保障。
    • 王越甲; 杨炜峰; 车路平; 王小珂; 卢胜明
    • 摘要: 目的 利用微卫星位点在不同近交系小鼠、大鼠中可能具有的多态性,分别筛选出可以一次性区别5种常用近交系小鼠和3种常用近交系大鼠的微卫星引物组合,为近交系小鼠和大鼠的遗传监测分析提供高效的微卫星位点与简易可靠的鉴定条件.方法 通过查询相关数据库以及文献报道,按照已有明确的微卫星实验数据记录为原则,进行比对和筛选,组成了小鼠37个和大鼠24个微卫星位点库.通过PCR扩增和5%琼脂糖电泳鉴定,优化筛选出可以区分单一品系和所有品系的微卫星位点组合.结果 小鼠优选出11个位点可用于5种常用近交系的鉴定,并建立了1个包含6个微卫星位点的库可用于5种小鼠品系的遗传监测;大鼠优选出6个微卫星位点,可用于3种常用近交系的监测.结论 成功建立了一套可以区分常用5种小鼠和3种大鼠品系的微卫星监测方法.
    • 崔智; 李晓娟; 白云峰; 侯俊; 戴广海; 李瑞生
    • 摘要: Objective To determine the genetic characteristics of PLCe knockout mice by polymorphic microsatellite DNA loci analysis. Methods Genome DNA of 28 PLCe gene knockout mice were amplified by PCR using screening 15 microsatellite DNA loci, and population genetic diversity was identified by gene fragments. Results Among 13 microsatellite DNA loci (DlMit365, D3Mit51 , D4Mit235 , D6MM02, D7Mit281 , D8Mitll3, D9Mit23 , D10MM80, D13Mit88,D16Mitl45,D17Mit36,D18Mit94,D19Mit97),each locus of electrophoresis distance of DNA fragments in the 28 PLCe gene knockout mice kept consistent and presented monomorphism , indicating the genetic stability. However the two loci Dq (knock genotype) and Dy (wild-type) were used to discriminate 28 PLCe gene knockout mice by PCR amplification. Among them, 6 mice were of gene knock mice, 7 mice were of wild-type mice, and 15 mice were of heterozygous type. Conclusions The method of microsatellite marker analysis can be used to monitor population genetic quality and accurately distinguish different genotypes of mice , providing a feasible method for the detection of genetic quality%目的 利用多态性微卫星DNA位点分析PLCε基因敲除小鼠的遗传特性.方法 用所筛选的15个微卫星DNA位点对28只PLCε基因敲除小鼠的DNA进行了PCR扩增,通过基因片段大小来分析群体的遗传多样性.结果 13个微卫星DNA位点中(D1Mit365、D3Mit51、D4Mit235、D6Mit102、D7Mit281、D8Mit113、D9Mit23、D10Mit180、D13Mit88、D16Mit145、D17Mit36、D18Mit94、D19Mit97)每个位点的28只小鼠DNA片段泳动距离一致,呈现单态性,表明该群体符合近交系的遗传特性;而利用Dq(敲基因型)和Dy(野生型)两个位点对28只小鼠的PCR扩增结果进行了鉴别分析,其中敲除基因型小鼠为6只;野生型为7只;杂合型为15只.结论 利用微卫星标记技术可以对群体进行遗传质量监测,并能有效地鉴别不同的基因型,为小鼠的遗传质量监测提供了一种可行的方法.
    • 郑龙; 李建辉; 王俊霞; 刘军须; 蔡月花; 连伟光; 刘福英
    • 摘要: Objective The polymorphism of microsatellite loci among four inbred strains of rats ( HFJ, MU, Lewis, F344) was analyzed using 24 pair primers. Methods To extract the genomic DNA of MU, HFJ, Lewis and F344 inbred rats by standard extraction procedure with phenol/chloroform. 24 microsatellite loci were selected for PCR amplification. Through native polyacrylamide gel eleclrophoresis and silver staining, microsatellite polymorphism was analyzed in the four strains of inbred rats. Results The amplified products of 24 microsatellite loci among different individual rats of these 4 strains and the same strain rats displayed single allelic gene band. Among the 24 microsatellite loci, it displayed mono-morphism between HFJ and MU rats. But HFJ and MU rats had significant deviations from Lewis and F344 rats. 14 micro-satellite loci turned out to be polymorphic, and 10 microsatellite loci displayed monomorphism. Conclusions The results of this study suggest that MU and HFJ strains basically meet the requirement of inbred strain. The 14 screened loci can be used for genetic monitoring of these inbred rats.%目的 用24对引物对近交系HFJ和MIJ大鼠的微卫星位点进行多态性分析,并选用近交系Lewis和F344大鼠作为对照,进行比较分析.方法 用传统的酚-氯仿法分别提取4个近交系大鼠MIJ、HFJ、Lewis和F344 的基因组DNA,选取大鼠24个微卫星位点,通过PCR扩增,扩增产物经过非变性聚丙烯酰胺凝胶电泳和银染,根据电泳结果,比较分析4种品系近交系大鼠之间微卫星多态性.结果 4种品系及品系内不同个体的近交系大鼠在24个微卫星位点上的扩增产物均出现一个条带,MIJ和HFJ大鼠在品系间和品系内均表现为单态性,同Lewis 和F344的扩增结果比较,14个位点显示多态性,有10个位点显示单态性.结论 两个近交系大鼠品系MIJ和HFJ符合近交系要求,筛选出的14个多态性微卫星位点可用于有关近交系大鼠的遗传背景监测.
    • 李瑞生; 李晓娟; 高蓉; 白云峰; 鲍龙涛; 战大伟
    • 摘要: 目的 研究国内食蟹猴种群的遗传背景特性,建立食蟹猴种群遗传质量监测方法.方法 采用微卫星DNA遗传标记技术对50只食蟹猴种群个体进行遗传质量监测及DNA多态性分析.结果 从100个微卫星DNA位点中筛选出20个多态性高的位点,其食蟹猴种群个体的等位基因数目为5-10条,个体间均呈现高度的多态性;其观察等位基因数(Na)为5.0~10.0,有效等位基因数(Ne)为4.6118~8.3404,基因多样性(H)为0.7832~0.8801和香隆信息指数(I)为1.5651~2.1592.结论 本实验有效地分析了食蟹猴种群的遗传多态性,为今后筛选特异性微卫星位点来建立食蟹猴种群遗传质量监测方法提供了理论依据.%Objective To investigate the genetic background features of Macaca fascicularis and to establish the genetic quality monitoring method in Macaca fascicularis. Methods Using microsatellite DNA genetic markers, genetic quality monitoring and DNA polymorphism analysis were conducted in 50 Macaca fascicularis. Results 20 loci with high polymorphism was selected from 100 microsatellite DNA loci, among which allele numbers in Macaca fascicularis were 5-10, and individuals showed a high degree of polymorphism. Observed number of alleles (Na) was 5.0 ~ 10.0, effective number of alleles (Ne) was 4.6118 ~ 8.3404, gene diversity ( H ) was 0. 7832 ~ 0. 8801 and Shannon's information index (I) was 1. 5651 ~2. 1592. Conclusions The experiments effectively analyzed the genetic diversity of Macacafascicularis,and it can provide a theoretical foundation for establishing genetic quality monitoring method with specific microsatellite loci selected in Macaca fascicularis.
    • 刘先菊; 黄馨; 王艳蓉; 刘云波
    • 摘要: 目的 用生化标记和微卫星检测法观察近交系C57BL/6J小鼠胚胎冷冻后的遗传质量是否发生改变.方法 采用国标(GB/T 14927.1-2008)生化标记基因检测法对4只玻璃化冷冻、解冻移植后获得的C57BL/6J F1代小鼠的13个生化位点进行预检测,再根据微卫星DNA多态性的遗传监测方法 进行多态性分析.结果 4只玻璃化冷冻后移植获得的C57BL/6 F1代小鼠的遗传概貌与国标(GB/T 14927.1-2008)中的14个生化位点完全一致;并与本文作者已建立"几种常用近交系小鼠微卫星DNA多态性的分析研究"的遗传监测结果 完全相符.结论 玻璃化冷冻法(EFS40,straw)对C57BL/6J冷冻胚胎小鼠的遗传物质并未造成影响,其遗传特性保持稳定,是可行的小鼠胚胎冷冻方法 .
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