Lycopene is one of the most abundant carotenoid w hich has multiple activities . In order to promote the application of lycopene in medicine , its antioxidant effect is discussed in this papper . The reducing activity , scavenging effects of both hydroxyl radical and superoxide anion , protections against the lipid peroxidation are measured by potassium ferricyanide method , deoxyribose degradation method , autoxidation of pyrogallol method and thiobarbituric acid colouration method , respectively . The detection of and oxidative damage of cells , protein , DNA are done by MTT method , SDS‐PAGE , capillary electrophoresis ,and all the test are compared with quercetin . The results indicate that lycopene shows some reducing activity and powerful ability of scavenging on free radicals . The EC50 for the elimination of hydroxyl radical and superoxide anion are 0.02 and 69.31 μmol · L -1 , respectively . Lycopene show s inhibiting effect of lipid peroxidation with IC 50 value of 20.8 μmol · L -1 and obviously protective effect on cells and DNA from H2 O2‐induced oxidative damage at the concentration of 5 μmol · L -1 . Also , lycopene is more active than quercetin in these systems .%番茄红素是最主要的类胡萝卜素，且具有多种生理活性．为了促进番茄红素在医药方面的应用，通过铁氰化钾法、脱氧核糖降解法、邻苯三酚自氧化法以及硫代巴比妥酸比色法检测番茄红素的还原力，以及对羟自由基、超氧阴离子的清除作用和对脂质过氧化的抑制作用；用M T T法、SDS‐PAGE法以及毛细管电泳检测番茄红素对细胞、蛋白质以及DNA氧化损伤的保护作用，用槲皮素为对照，研究番茄红素的抗氧化活性．结果表明，番茄红素具有一定的还原力和较强的自由基清除能力，对羟自由基和超氧阴离子的EC50分别为0.02和69.31μmol·L －1；抑制脂质过氧化的IC50为20.8μmol·L －1，在浓度为5μmol·L －1时对细胞、DNA的氧化损伤具有明显保护作用，且活性均比槲皮素强．表明番茄红素具有较强抗氧化活性．
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