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首页> 外文期刊>Pathobiology: journal of immunopathology, molecular and cellular biology >Role of Glycoproteins Isolated from Epicoccum purpurascens in Host-Pathogen Interaction.
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Role of Glycoproteins Isolated from Epicoccum purpurascens in Host-Pathogen Interaction.

机译：分离自紫葡萄球菌的糖蛋白在宿主-病原体相互作用中的作用。

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OBJECTIVE: Attachment to host matrix is an important provisory step for the institution of any fungal infection. The present study investigates the role of glycoproteins of Epicoccum purpurascens in host-fungal adherence. METHODS:Epicoccum spore-mycelial extract was fractionated on a concanavalin A-Sepharose column. Three glycoproteins of 12, 17 and 33 kDa (Epi p 1) were electroeluted and checked for hemagglutination and hemagglutination inhibition. The monosaccharide content of the highly potent protein Epi p 1 was determined by high-performance anion exchange chromatography and pulsed amperometric detection. The interaction of Epi p 1 with mannose-binding lectin (MBL) leading to the activation of the complement system was studied by immunoblot, ELISA and ELISA inhibition techniques. Immunoblot and immunoblot inhibition were carried out with culture filtrate to determine the nature of Epi p 1. RESULTS: 33 (Epi p 1)-, 17- and 12-kDa proteins were 58, 46 and 38 times more potent than crude extract in hemagglutination activity (HA). The HA of Epi p 1 was inhibited by N-acetyl glucosamine, glucose and laminin. Epi p 1 had a high mannose content, showed MBL binding in ion-dependent manner and caused complement activation. The protein was detected in culture filtrate and thus seems to play a significant role in fungal invasion. CONCLUSION: Epi p 1, an allergenic glycoprotein of E. purpurascens, is involved in host-fungal interactions through MBL.
机译：目的：对宿主基质的附着是任何真菌感染发生的重要重要步骤。本研究调查了表皮紫癜糖蛋白在宿主真菌粘附中的作用。方法：在伴刀豆球蛋白A-Sepharose柱上分级分离细小孢子孢子菌丝体提取物。分别洗脱12、17和33 kDa的三种糖蛋白（Epi p 1）并检查血凝和血凝抑制作用。通过高效阴离子交换色谱和脉冲安培检测法测定高效蛋白Epi p 1的单糖含量。通过免疫印迹，ELISA和ELISA抑制技术研究了Epi p 1与甘露糖结合凝集素（MBL）相互作用，从而激活补体系统。用培养滤液进行免疫印迹和免疫印迹抑制，以确定Epi p 1的性质。结果：在血凝反应中，33（Epi p 1）-，17-和12-kDa蛋白的效力是粗提物的58、46和38倍活动（HA）。 Epi p 1的HA被N-乙酰氨基葡萄糖，葡萄糖和层粘连蛋白抑制。 Epi p 1具有高甘露糖含量，以离子依赖性方式显示MBL结合并引起补体激活。在培养滤液中检测到该蛋白质，因此似乎在真菌侵袭中起重要作用。结论：Epi p 1是紫癜大肠杆菌的一种过敏原糖蛋白，通过MBL参与宿主-真菌相互作用。

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