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首页> 外文期刊>Separation and Purification Technology >Continuous ultrafiltration membrane reactor coupled with nanofiltration for the enzymatic synthesis and purification of galactosyl-oligosaccharides
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Continuous ultrafiltration membrane reactor coupled with nanofiltration for the enzymatic synthesis and purification of galactosyl-oligosaccharides

机译：连续超滤膜反应器与纳滤结合用于半乳糖基寡糖的酶促合成和纯化

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摘要

Continuous enzymatic production of galactosyl-oligosaccharides (GOS) from a lactose substrate in an ultrafiltration membrane reactor (UMR) coupled with a nanofiltration separation system (CPNSS) was investigated. The overall rate of production over 4 h of continuous production was 80-104 mg GOS formed/U with an average residence time of 66 min, an initial lactose concentration of 300 g/L, an inlet pressure of 2.0 bar inlet pressure, and an outlet pressure of 1.5 bar. In the continuous diafiltration (CD) process, the concentration of various sugars and the relationship between the yield and purity of oligosaccharides was well predicted by mathematical models, the increased rate of sugar rejections was less than 10%, and the decreased rate of concentrations in the tank was less than 15%. In the CPNSS, 33.4 wt.% GOS was obtained in the UMR, and 1.24 kg of high-purity GOS (specifically, GOS purity of 57.2% and lactose content less than 20%) was achieved. This final yield was 80.1% GOS, which meets industry standards. (C) 2015 Elsevier B.V. All rights reserved.
机译：研究了在超滤膜反应器（UMR）与纳滤分离系统（CPNSS）结合的情况下，从乳糖底物连续酶促生产半乳糖基寡糖（GOS）。连续生产4小时后的总生产速率为80-104 mg GOS形成/ U，平均停留时间为66分钟，初始乳糖浓度为300 g / L，入口压力为2.0 bar入口压力，并且出口压力为1.5 bar。在连续渗滤（CD）过程中，通过数学模型可以很好地预测各种糖的浓度以及寡糖的产量与纯度之间的关系，糖去除率的增加率低于10％，而糖的浓度下降率则较低。战车少于15％在CPNSS中，在UMR中获得了33.4 wt。％的GOS，并获得了1.24 kg的高纯度GOS（具体而言，GOS纯度为57.2％，乳糖含量小于20％）。最终产率为80.1％GOS，符合行业标准。 （C）2015 Elsevier B.V.保留所有权利。

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