摘要：
Objective To study the protective effect of Guiqi Baizhu Recipe(GBR) on cisplatin-induced hepatotoxicity in mice. Methods Mice were randomly divided into five groups: normal group,model group,experimental-L,M and H groups,with 10 mice in each group. The mice hepatotoxicity model were preparated by injected intraperitoneally with 2. 0 mg·mL-1 of cisplatin injection 0. 2 mL. At the same time of model,the mice in three doses experimental groups were intragastric administration GBR 4. 1,8. 2,16. 4 g·kg-1; the mice in normal and model groups were intragastric administration the same volume of distilled water,1 times a day for 28 days. The blood routine in mice were detected by automatic hematology analyzer. The alanine transaminase (ALT) ,aspartate transaminase(AST) activity in blood of the liver tissue were detected by fully automatic chemistry analyzer. Results The WBC in normal group,model group,experimental-L,- M and-H groups respectively were(12. 48 ± 1. 67) ,(4. 37 ± 0. 30) ,(6. 36 ± 0. 48) ,(7. 63 ± 0. 08) ,(7. 46 ± 1. 46) 109/L-1; the PLT in the five groups respectively were (993. 67 ± 173. 34) ,(629. 67 ± 134. 11) ,(839. 67 ± 35. 81) ,(889. 50 ± 81. 32) ,(918. 00 ± 49. 37) g·L-1; comparison between the model group with normal group,with WBC and PLT decreased, the difference was significant (all P < 0. 01); comparison experimental-L,- M and-H groups with model group,with WBC and PLT increased,the difference was significant (P < 0. 05,P < 0. 01). The ALT in the five groups respectively were (44. 33 ± 14. 36) ,(77. 33 ± 16. 77) ,(61. 00 ± 8. 19) ,(55. 33 ± 7. 51) ,(53. 33 ± 10. 26) U·L-1; the AST in the five groups respectively were (145. 33 ± 19. 35) ,(205. 67 ± 33. 26) ,(196. 67 ± 35. 12) ,(163. 00 ± 11. 27) ,(156. 33 ± 21. 55) U·L-1; comparison between the model group with normal group, the difference was significant with ALT and AST increase (all P < 0. 01); comparison experimental-H group with model group, the difference was significant with ALT and AST decrease (all P < 0. 05). Conclusion GBR can reduce the liver toxicity induced by cisplatin in mice.%目的 研究归芪白术方(GBR)对顺铂所致肝毒性的保护作用.方法 按照体重将小鼠随机分为5组:空白组,模型组和低、中、高3个剂量实验组,每组10只.以腹腔注射2.0 mg·mL-1顺铂注射液0.2 mL制备小鼠肝毒性模型.造模的同时,低、中、高3个剂量实验组灌胃GBR4.1,8.2,16.4 g·kg-1;空白组和模型组灌胃等体积蒸馏水,1次/1 d,连续28 d.用全自动血球分析仪检测小鼠血常规,以全自动生化分析仪检测肝组织中谷草转氨酶(AST)和谷丙转氨酶(ALT)活性.结果 空白组、模型组和低、中、高3个剂量实验组的WBC分别为(12.48±1.67),(4.37±0.30),(6.36±0.48),(7.63±0.08)和(7.46±1.46)109/L-1;这5组的PLT分别为(993.67±173.34),(629.67±134.11),(839.67±35.81),(889.50±81.32)和(918.00±49.37)g·L-1,模型组与空白组比较,WBC和PLT均明显降低,差异均有统计学意义(均P<0.01);低、中、高3个剂量实验组与模型组比较,WBC和PLT均明显增加,差异均有统计学意义(P<0.05或P<0.01).这5组的ALT分别为(44.33±14.36),(77.33±16.77),(61.00±8.19),(55.33±7.51)和(53.33±10.26)U·L-1;这5组的AST分别为(145.33±19.35),(205.67±33.26),(196.67±35.12),(163.00±11.27)和(156.33±21.55)U·L-1,模型组与空白组比较,ALT和AST均明显增加,差异均有统计学意义(均P<0.01);高剂量实验组与模型组比较,ALT和AST均明显降低,差异均有统计学意义(均P<0.05).结论 GBR能够减弱顺铂对小鼠的肝毒性作用.
